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Chiral and achiral separation using in-house coated β-cyclodextrin column, comparison with commercially available β-cyclodextrin column
Procházková, Hana ; Kalíková, Květa (advisor) ; Kozlík, Petr (referee)
The main aim of this work was to prepare a new chiral stationary phase by dynamic coating of an ion-exchange stationary phase by PEMPDA-β-cyclodextrin (N1 , N1 , N3 , N3 , N3 -pentamethylpropane-1,3-diamine-β-cyclodextrin) and to test column selectivity and separation abilities of the newly prepared sorbent for chiral and also achiral sets of analytes and to evaluate stability of the column. Commercial β- cyclodextrin stationary phase (Astec Cyclobond I 2000 column) was used for comparison. The set of chiral analytes contained five structurally different compounds - lorazepam, oxazepam, BNP, HDDP and Cl-HDDP. Six different groups of achiral analytes were used for testing the column selectivity. All measurements were carried out in reversed phase separation mode. Methanol and 10mM ammonium acetate (pH 4.00) were used as mobile phase constituents. Baseline separations of oxazepam enantiomers and five mixtures of achiral analytes were achieved with PEMPDA-β-cyclodextrin stationary phase. Partial separations of two chiral analytes (HDDP and Cl-HDDP) and one mixture of achiral analytes were obtained with this stationary phase. Enantiomers of BNP and oxazepam and four mixtures of achiral analytes were baseline separated on the Astec Cyclobond I 2000 column. Partial separations were obtained for the rest...
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